CHEM281

Focus on the combined use of organic chemistry and molecular biology to make, manipulate and measure biomacromolecules, with special focus on DNA and RNA. Synthetic and enzymatic methods for design and construction of oligonucleotides and nucleic acids; methods for bioconjugation and labeling; fluorescence tools; intracellular delivery strategies; selection and evolution methods; CRISPR mechanisms. Prerequisite: One year of undergraduate organic chemistry. Completion of a course in molecular biology is strongly recommended.